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K. Murugaiah ¹, R. Kesavan ¹, T. S. Dhanaraj ²

Affiliations
1 PG and Research Department of Biochemistry, Enathi Rajappaa College, Enathi, Pattukkottai, Thanjavur Dt., Tamilnadu, IN
2 34 Chellapillaiyar Kovil Street, Karanthai, Thanjavur -613002, IN

Abstract

Enzymes are considered as nature’s catalysis most enzymes today (and in future) produced from the microorganisms. In the present study, Bacillus subtilis and Pseudomonas aeruginosa were isolated from the soil samples. The isolated organisms were identified using standard microbiological methods. The inoculums were prepared with waste substrate (sugarcane bagasse). The isolated bacterial strain were inoculated in the medium and incubated at 30°C for 3 days. The high-level amylase production was observed in Bacillus subtilis inoculated medium so this strain used for the optimization studies. The amylase activity was optimized in various physical parameters such as temperature, pH and chemical parameters like nitrogen and carbon sources. The maximum production of amylase was recorded at 35° C and pH 7.0. The amylase production by Bacillus subtilis was recorded maximum in starch-supplemented medium when compared to other carbon sources (maltose and glucose). Among the various nitrogen sources (Ammonium sulphate, ammonium chloride and ammonium nitrate) used, Ammonium sulphate supplemented medium showed the significant results in the production of amylase by Bacillus subtilis.

Keywords

Amylase, Sugarcane Bagasse, Bacillus Subtilis, Pseudomonas aeruginosa and Submerged Fermentation.

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A. Dominic Amalraj ¹, C. Parkavi ¹, K. Murugaiah ¹, T. S. Dhanaraj ¹

Affiliations
1 Dept. of Biochemistry, Enathi Rajappa College, Pattukkottai, Thanjavur Dt. Tamil Nadu, Pin: 614 615, IN

Abstract

The objective of the present study is to evaluate the hypolipidemic activity of Cyperous rotundus on CCl₄ induced Dyslipemia in rat. The aqueous extract of Cyperous rotundus significantly show the hypolipidimic activation through lipid profile liver marker enzyme.

Keywords

Hypolipidemic Activity, Cyperous rotundus, CCl₄ Induced.

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T. S. Dhanaraj ¹, R. Gowthami ¹, S. Rajlakshmi ¹, K. Murugaiah ¹

Affiliations
1 PG & Research Department of Biochemistry, Enathi Rajappaa College, Enathi, Pattukkottai, Thanjavur Dt., Tamil Nadu, 614 615, IN

Abstract

The objective of this study was to investigate the hepatoprotective activity of crude aqueous extract of leaves of Hygrophila auriculata against CCl4 induced hepatotoxicity. The leaves was dried in shade, they were powdered and Extracted with aqueous. The biochemical markers of hepatic damage like tested as MDA, GSH, Protein, Bilirubin, SGOT, ALP and SGPT recorded a significant alteration in CCl4 treated rats. However treatment with Hygrophila auriculata restored the level to near normal was observed. The potential hepatoprotective activity of Hygrophila auriculata is due to the presence of phytochemical constitution such as flavonoids and polyphenolic compounds present in plant.

Keywords

Hepatoprotective, CCl₄-Induced, Hygrophila auriculata.

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T. S. Dhanaraj ¹, K. Murugaiah ¹, Sarmila ¹

Affiliations
1 PG and Research Dept. of Biochemistry, Enathi Rajappa College, Pattukkottai, Thanjavur Dt. Tamil Nadu, Pin: 614 615, IN

Abstract

The objective of this study was to investigate the Anti-ulcer activity of crude aqueous extract of leaves of Mukia maderasapatana against stress induced. The leaves was dried in shade, they were powdered and Extracted with aqueous. The biochemical markers of ulcer like MDA, GSH, gastric juice, volume and pH were recorded a significant alteration under stress condition in rats. However treatment with Mukia maderasapatana restored significantly to the normal level.

Keywords

Anti-Ulcer, Stress Induced, Mukia maderaspatana.

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